Prefabrication of a vascularized allograft bone in a recipient rat implantation of a flow-through vascular bundle

Introduction: A previous study attempted to prefabricate a vascularized allograft bone in a recipient rat. In this model, the saphenous vascular bundles of recipient rats were transected and implanted into the transplanted donor bones. Although blood circulation had been established in the transplanted vascularized bones and bleeding from the bones was observed at the time of bone collection, occasional occlusions of vascular bundles were observed in the deep area of transplanted bone., In the current study the vascular bundle was implanted in a flowthrough manner in order to improve this problem. In addition, this study evaluated whether the combined treatment with bone morphogenetic protein and bisphosphonate is effective for the stimulation of bone formation and the inhibition of bone resorption in the transplanted bone.